Review



injection microcapillary holder  (Eppendorf AG)


Bioz Verified Symbol Eppendorf AG is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 95
      Buy from Supplier

    Structured Review

    Eppendorf AG injection microcapillary holder
    A. Injection and electroporation of pCMV-IRES-GFP into the ES at stage HH16. Light green triangle represents the injection <t>microcapillary</t> and dark green dots represent where DNA was injected. Black and red bars indicate the position of the −ve and +ve electrodes respectively in relation to the ES. B. Example of an embryo electroporated with IRES-GFP at stage HH16 then incubated for 24 h, strong GFP fluorescence can be seen in somites. C. Enlarged electroporated somites of the embryo in (B). D. Embryo electroporated with IRES-GFP at stage HH16 and incubated for 72 h, strong GFP fluorescence signals can be seen in the somites. E. Embryo electroporated with IRES-RFP at stage HH16 and incubated for 72 h, RFP strong fluorescence signals can be seen in the somites. F. Transverse section of the embryo in (E) after processing for double ISH showing RFP (red) expression in the transfected somite domain (arrows) and MyoD expression (blue) in both injected (right) and non-injected control sides (left). GFP in (B-D) was detected by Alexa Fluor 488 ηm and RFP in (E) by Alexa Fluor 565 ηm fluorescence filters. av: auditory vesicle; e: eye; ES: epithelial somites; fb: fore-brain; fl: fore-limb; h: heart; hb: hind-brain; hl: hind limb; mb: mid-brain; nt: neural tube; psm: presegmented mesoderm. Scale bars: 0.5 mm in (A and D), 1.5 mm in (B), 0.4 mm (C), 0.3 mm in (E) and 0.1 mm in (F).
    Injection Microcapillary Holder, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 95/100, based on 442 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/injection+microcapillary+holder/pmc06706099-52-82-114?v=Eppendorf+AG
    Average 95 stars, based on 442 article reviews
    injection microcapillary holder - by Bioz Stars, 2026-07
    95/100 stars

    Images

    1) Product Images from "Use of chick neural tube for optimizing the PSM and epithelial somites electroporation parameters: A detailed protocol"

    Article Title: Use of chick neural tube for optimizing the PSM and epithelial somites electroporation parameters: A detailed protocol

    Journal: Journal of Biological Methods

    doi: 10.14440/jbm.2018.253

    A. Injection and electroporation of pCMV-IRES-GFP into the ES at stage HH16. Light green triangle represents the injection microcapillary and dark green dots represent where DNA was injected. Black and red bars indicate the position of the −ve and +ve electrodes respectively in relation to the ES. B. Example of an embryo electroporated with IRES-GFP at stage HH16 then incubated for 24 h, strong GFP fluorescence can be seen in somites. C. Enlarged electroporated somites of the embryo in (B). D. Embryo electroporated with IRES-GFP at stage HH16 and incubated for 72 h, strong GFP fluorescence signals can be seen in the somites. E. Embryo electroporated with IRES-RFP at stage HH16 and incubated for 72 h, RFP strong fluorescence signals can be seen in the somites. F. Transverse section of the embryo in (E) after processing for double ISH showing RFP (red) expression in the transfected somite domain (arrows) and MyoD expression (blue) in both injected (right) and non-injected control sides (left). GFP in (B-D) was detected by Alexa Fluor 488 ηm and RFP in (E) by Alexa Fluor 565 ηm fluorescence filters. av: auditory vesicle; e: eye; ES: epithelial somites; fb: fore-brain; fl: fore-limb; h: heart; hb: hind-brain; hl: hind limb; mb: mid-brain; nt: neural tube; psm: presegmented mesoderm. Scale bars: 0.5 mm in (A and D), 1.5 mm in (B), 0.4 mm (C), 0.3 mm in (E) and 0.1 mm in (F).
    Figure Legend Snippet: A. Injection and electroporation of pCMV-IRES-GFP into the ES at stage HH16. Light green triangle represents the injection microcapillary and dark green dots represent where DNA was injected. Black and red bars indicate the position of the −ve and +ve electrodes respectively in relation to the ES. B. Example of an embryo electroporated with IRES-GFP at stage HH16 then incubated for 24 h, strong GFP fluorescence can be seen in somites. C. Enlarged electroporated somites of the embryo in (B). D. Embryo electroporated with IRES-GFP at stage HH16 and incubated for 72 h, strong GFP fluorescence signals can be seen in the somites. E. Embryo electroporated with IRES-RFP at stage HH16 and incubated for 72 h, RFP strong fluorescence signals can be seen in the somites. F. Transverse section of the embryo in (E) after processing for double ISH showing RFP (red) expression in the transfected somite domain (arrows) and MyoD expression (blue) in both injected (right) and non-injected control sides (left). GFP in (B-D) was detected by Alexa Fluor 488 ηm and RFP in (E) by Alexa Fluor 565 ηm fluorescence filters. av: auditory vesicle; e: eye; ES: epithelial somites; fb: fore-brain; fl: fore-limb; h: heart; hb: hind-brain; hl: hind limb; mb: mid-brain; nt: neural tube; psm: presegmented mesoderm. Scale bars: 0.5 mm in (A and D), 1.5 mm in (B), 0.4 mm (C), 0.3 mm in (E) and 0.1 mm in (F).

    Techniques Used: Injection, Electroporation, Incubation, Fluorescence, Expressing, Transfection, Control



    Similar Products

    microcapillary tubing  (Narishige inc)
    86
    Narishige inc microcapillary tubing
    Microcapillary Tubing, supplied by Narishige inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/injection+microcapillary+holder/pm29093206-26-20-22?v=Narishige+inc
    Average 86 stars, based on 1 article reviews
    microcapillary tubing - by Bioz Stars, 2026-07
    86/100 stars
    		  Buy from Supplier
    injection microcapillary holder  (Eppendorf AG)
    95
    Eppendorf AG injection microcapillary holder
    A. Injection and electroporation of pCMV-IRES-GFP into the ES at stage HH16. Light green triangle represents the injection <t>microcapillary</t> and dark green dots represent where DNA was injected. Black and red bars indicate the position of the −ve and +ve electrodes respectively in relation to the ES. B. Example of an embryo electroporated with IRES-GFP at stage HH16 then incubated for 24 h, strong GFP fluorescence can be seen in somites. C. Enlarged electroporated somites of the embryo in (B). D. Embryo electroporated with IRES-GFP at stage HH16 and incubated for 72 h, strong GFP fluorescence signals can be seen in the somites. E. Embryo electroporated with IRES-RFP at stage HH16 and incubated for 72 h, RFP strong fluorescence signals can be seen in the somites. F. Transverse section of the embryo in (E) after processing for double ISH showing RFP (red) expression in the transfected somite domain (arrows) and MyoD expression (blue) in both injected (right) and non-injected control sides (left). GFP in (B-D) was detected by Alexa Fluor 488 ηm and RFP in (E) by Alexa Fluor 565 ηm fluorescence filters. av: auditory vesicle; e: eye; ES: epithelial somites; fb: fore-brain; fl: fore-limb; h: heart; hb: hind-brain; hl: hind limb; mb: mid-brain; nt: neural tube; psm: presegmented mesoderm. Scale bars: 0.5 mm in (A and D), 1.5 mm in (B), 0.4 mm (C), 0.3 mm in (E) and 0.1 mm in (F).
    Injection Microcapillary Holder, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/injection+microcapillary+holder/pmc06706099-52-82-114?v=Eppendorf+AG
    Average 95 stars, based on 1 article reviews
    injection microcapillary holder - by Bioz Stars, 2026-07
    95/100 stars
    		  Buy from Supplier
    microcapillary holder  (Narishige inc)
    94
    Narishige inc microcapillary holder
    A. Injection and electroporation of pCMV-IRES-GFP into the ES at stage HH16. Light green triangle represents the injection <t>microcapillary</t> and dark green dots represent where DNA was injected. Black and red bars indicate the position of the −ve and +ve electrodes respectively in relation to the ES. B. Example of an embryo electroporated with IRES-GFP at stage HH16 then incubated for 24 h, strong GFP fluorescence can be seen in somites. C. Enlarged electroporated somites of the embryo in (B). D. Embryo electroporated with IRES-GFP at stage HH16 and incubated for 72 h, strong GFP fluorescence signals can be seen in the somites. E. Embryo electroporated with IRES-RFP at stage HH16 and incubated for 72 h, RFP strong fluorescence signals can be seen in the somites. F. Transverse section of the embryo in (E) after processing for double ISH showing RFP (red) expression in the transfected somite domain (arrows) and MyoD expression (blue) in both injected (right) and non-injected control sides (left). GFP in (B-D) was detected by Alexa Fluor 488 ηm and RFP in (E) by Alexa Fluor 565 ηm fluorescence filters. av: auditory vesicle; e: eye; ES: epithelial somites; fb: fore-brain; fl: fore-limb; h: heart; hb: hind-brain; hl: hind limb; mb: mid-brain; nt: neural tube; psm: presegmented mesoderm. Scale bars: 0.5 mm in (A and D), 1.5 mm in (B), 0.4 mm (C), 0.3 mm in (E) and 0.1 mm in (F).
    Microcapillary Holder, supplied by Narishige inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/injection+microcapillary+holder/10__1023_slash_b_ascii58_mics__0000006846__13226__38-48-30-40?v=Narishige+inc
    Average 94 stars, based on 1 article reviews
    microcapillary holder - by Bioz Stars, 2026-07
    94/100 stars
    		  Buy from Supplier
    microcapillary  (Narishige inc)
    94
    Narishige inc microcapillary
    A. Injection and electroporation of pCMV-IRES-GFP into the ES at stage HH16. Light green triangle represents the injection <t>microcapillary</t> and dark green dots represent where DNA was injected. Black and red bars indicate the position of the −ve and +ve electrodes respectively in relation to the ES. B. Example of an embryo electroporated with IRES-GFP at stage HH16 then incubated for 24 h, strong GFP fluorescence can be seen in somites. C. Enlarged electroporated somites of the embryo in (B). D. Embryo electroporated with IRES-GFP at stage HH16 and incubated for 72 h, strong GFP fluorescence signals can be seen in the somites. E. Embryo electroporated with IRES-RFP at stage HH16 and incubated for 72 h, RFP strong fluorescence signals can be seen in the somites. F. Transverse section of the embryo in (E) after processing for double ISH showing RFP (red) expression in the transfected somite domain (arrows) and MyoD expression (blue) in both injected (right) and non-injected control sides (left). GFP in (B-D) was detected by Alexa Fluor 488 ηm and RFP in (E) by Alexa Fluor 565 ηm fluorescence filters. av: auditory vesicle; e: eye; ES: epithelial somites; fb: fore-brain; fl: fore-limb; h: heart; hb: hind-brain; hl: hind limb; mb: mid-brain; nt: neural tube; psm: presegmented mesoderm. Scale bars: 0.5 mm in (A and D), 1.5 mm in (B), 0.4 mm (C), 0.3 mm in (E) and 0.1 mm in (F).
    Microcapillary, supplied by Narishige inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/injection+microcapillary+holder/10__1023_slash_b_ascii58_mics__0000006846__13226__38-48-25-40?v=Narishige+inc
    Average 94 stars, based on 1 article reviews
    microcapillary - by Bioz Stars, 2026-07
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    A. Injection and electroporation of pCMV-IRES-GFP into the ES at stage HH16. Light green triangle represents the injection microcapillary and dark green dots represent where DNA was injected. Black and red bars indicate the position of the −ve and +ve electrodes respectively in relation to the ES. B. Example of an embryo electroporated with IRES-GFP at stage HH16 then incubated for 24 h, strong GFP fluorescence can be seen in somites. C. Enlarged electroporated somites of the embryo in (B). D. Embryo electroporated with IRES-GFP at stage HH16 and incubated for 72 h, strong GFP fluorescence signals can be seen in the somites. E. Embryo electroporated with IRES-RFP at stage HH16 and incubated for 72 h, RFP strong fluorescence signals can be seen in the somites. F. Transverse section of the embryo in (E) after processing for double ISH showing RFP (red) expression in the transfected somite domain (arrows) and MyoD expression (blue) in both injected (right) and non-injected control sides (left). GFP in (B-D) was detected by Alexa Fluor 488 ηm and RFP in (E) by Alexa Fluor 565 ηm fluorescence filters. av: auditory vesicle; e: eye; ES: epithelial somites; fb: fore-brain; fl: fore-limb; h: heart; hb: hind-brain; hl: hind limb; mb: mid-brain; nt: neural tube; psm: presegmented mesoderm. Scale bars: 0.5 mm in (A and D), 1.5 mm in (B), 0.4 mm (C), 0.3 mm in (E) and 0.1 mm in (F).

    Journal: Journal of Biological Methods

    Article Title: Use of chick neural tube for optimizing the PSM and epithelial somites electroporation parameters: A detailed protocol

    doi: 10.14440/jbm.2018.253

    Figure Lengend Snippet: A. Injection and electroporation of pCMV-IRES-GFP into the ES at stage HH16. Light green triangle represents the injection microcapillary and dark green dots represent where DNA was injected. Black and red bars indicate the position of the −ve and +ve electrodes respectively in relation to the ES. B. Example of an embryo electroporated with IRES-GFP at stage HH16 then incubated for 24 h, strong GFP fluorescence can be seen in somites. C. Enlarged electroporated somites of the embryo in (B). D. Embryo electroporated with IRES-GFP at stage HH16 and incubated for 72 h, strong GFP fluorescence signals can be seen in the somites. E. Embryo electroporated with IRES-RFP at stage HH16 and incubated for 72 h, RFP strong fluorescence signals can be seen in the somites. F. Transverse section of the embryo in (E) after processing for double ISH showing RFP (red) expression in the transfected somite domain (arrows) and MyoD expression (blue) in both injected (right) and non-injected control sides (left). GFP in (B-D) was detected by Alexa Fluor 488 ηm and RFP in (E) by Alexa Fluor 565 ηm fluorescence filters. av: auditory vesicle; e: eye; ES: epithelial somites; fb: fore-brain; fl: fore-limb; h: heart; hb: hind-brain; hl: hind limb; mb: mid-brain; nt: neural tube; psm: presegmented mesoderm. Scale bars: 0.5 mm in (A and D), 1.5 mm in (B), 0.4 mm (C), 0.3 mm in (E) and 0.1 mm in (F).

    Article Snippet: For setting up the electroporation station ( ): (1) Obtain a commercially available galvanized iron sheet with approximate measurements of 80 cm length, 40 cm width, and 3 mm thickness, and then place the iron sheet in the middle of the electroporation station; (2) Place the stereomicroscope with its associated reflected light units in the middle of the iron sheet; (3) Place a manual micromanipulator (loaded on a mounting magnetic base) on each side of the stereomicroscope, one micromanipulator should hold the injection microcapillary holder and another should carry the electrodes’ holder; switch the magnetic controls of the mounting bases “on” to fix them firmly in place on the iron sheet; (4) Place the Eppendorf microinjector ( ) on one side of the electroporation station and the electroporator (connected to the current amplifier) ( ) on the other side; and (5) Connect the injection microcapillary to the microinjector using the provided tubing and the electrodes to the electroporator using the provided electric wires.

    Techniques: Injection, Electroporation, Incubation, Fluorescence, Expressing, Transfection, Control